"> (Answered) Question 1: The hydropathy plot of a protein-X is shown in Figure below. To understand the organization of protein-X in the membrane, various hybrid...

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(Answered) Question 1: The hydropathy plot of a protein-X is shown in Figure below. To understand the organization of protein-X in the membrane, various hybrid...

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: The hydropathy plot of a protein-X is shown in Figure below.  

To understand the organization of protein-X in the membrane, various hybrid fusions of this protein were created as shown in the figure on the right. Fusion proteins were made with the membrane protein-X at the N-terminus and alkaline phosphatase (AP) at the C-terminus. AP activity of all the fusion proteins was measured and resulted activity is provided on in the figure (listed as high or low). Part of protein-X is represented as unshaded segment. AP is represented as shaded segment. Inverted V indicates the site from which various amino acids were deleted. Number for most c-terminus amino acid for protein-X is provided on the figure.

Please note:  1. AP has no hydrophobic domains. 2. Keep in mind the location of AP in a cell.

  • How many membrane domains are there in original protein-X? (1 points)
  • Based on data provided, predict how is the original protein organized in the membrane? (3 points)
  • Explain how the AP activity results support this arrangement. (3 points)
  • Explain why AP activity is low in some of the fusion proteins. (3 points)

 

Question 3:

To understand the role of various factor in nuclear import, an experiment was performed using GFP-tagged Ran (either WT Ran or mutant Ran-Q69L). Note: Q69L mutant can not hydrolyze GTP.

Which Ran was used

Nucleotide used

Cytoplasm added

Was Ran transported to nucleus

WT

GDP

No

No

WT

GDP

Yes

Yes

RanQ69L

GTP

No

No

RanQ69L

GTP

Yes

No

  • Based on the results, make three conclusions. (4.5 points)
  • Instead of using RanQ69L, could researchers have used Ran-GppNp? How would their results vary and how the conclusions will differ? (5.5 points)


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This question has been answered and updated today 10th May 2019

 

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